PMID:11134941
| Citation |
Lee, JE, Cornell, KA, Riscoe, MK and Howell, PL (2001) Expression, purification, crystallization and preliminary X-ray analysis of Escherichia coli 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase. Acta Crystallogr. D Biol. Crystallogr. 57:150-2 |
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| Abstract |
A recombinant form of Escherichia coli 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (E.C. 3.2.2.9) has been purified to homogeneity and crystallized using the hanging-drop vapour-diffusion technique. While several different crystallization conditions were obtained, only one set of conditions yielded crystals suitable for X-ray diffraction analysis. These crystals grow as diamond-shaped wedges, with unit-cell parameters a = 50.92, b = 133.99, c = 70.88 A, alpha = beta = gamma = 90 degrees. The crystals belong to space group P2(1)2(1)2 and diffract to a minimum d spacing of 2.3 A on a MAR345 image plate with a Rigaku RU-200 rotating-anode X-ray generator. On the basis of density calculations, two monomers are predicted per asymmetric unit (Matthews coefficient, V(M) = 2.37 A(3) Da(-1)), with a solvent content of 48%. |
| Links | |
| Keywords |
Amino Acid Sequence; Crystallization; Crystallography, X-Ray; Deoxyadenosines/chemistry; Escherichia coli/enzymology; Molecular Sequence Data; N-Glycosyl Hydrolases/chemistry; Thionucleosides/chemistry |
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