PMID:11129584
| Citation |
Nishiyama, K, Suzuki, H and Tokuda, H (2000) Role of the non-essential region encompassing the N-terminal two transmembrane stretches of Escherichia coli SecE. Biosci. Biotechnol. Biochem. 64:2121-7 |
|---|---|
| Abstract |
SecE is an essential component of the protein translocation machinery of Escherichia coli and has three transmembrane stretches. An N-terminal region (SecE-N) encompassing the first two transmembrane stretches is dispensable for protein translocation but a SecE derivative (SecE-C) lacking this region is very unstable. We show here that FtsH, the AAA (ATPases associated with diverse cellular activities) family protease, causes the instability of SecE-C. SecE-C became stable when SecE-N was co-expressed. Deletion of the N-terminal region of SecE also rendered the SecE-SecY-SecG complex unstable. In spite of these alterations, the N-terminal region of SecE had little stimulatory effect on protein translocation in vivo or in vitro. |
| Links | |
| Keywords |
Bacterial Proteins/chemistry; Escherichia coli/chemistry; Escherichia coli Proteins |
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