PMID:11127894

Citation	Craig, DB, Hall, T and Goltz, DM (2000) Escherichia coli beta-galactosidase is heterogeneous with respect to a requirement for magnesium. Biometals 13:223-9
Abstract	Commercially obtained E. coli beta-galactosidase was stored at 25 degrees C in buffer containing 1 mM MgCl2 and in buffer containing no added MgCl2. Samples were removed at set times and the activity of individual enzyme molecules assayed. When stored in the presence of 1 mM magnesium, the number of active molecules did not change over a 2.5-h period. When stored in the absence of added MgCl2, over half the enzyme molecules became inactive within the first hour. However, those molecules which retained activity remained active for the duration of the experiment. This indicates that there may exist two populations of E. coli beta-galactosidase, one which requires storage in the presence of the higher concentration of Mg2+ in order to remain active. There was no observed correlation between this requirement for magnesium and reaction rate. Additionally, the presence of the 1 mM MgCl2 was found to decrease the average activity of the beta-galactosidase molecules under the conditions employed.
Links	PubMed
Keywords	Bacterial Proteins/chemistry; Bacterial Proteins/metabolism; Buffers; Drug Storage; Electrophoresis, Capillary; Enzyme Stability; Escherichia coli/enzymology; Magnesium/chemistry; Magnesium/metabolism; Magnesium Chloride/chemistry; Protein Conformation; beta-Galactosidase/chemistry
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.