PMID:11122375
| Citation |
Taghbalout, A, Landoulsi, A, Kern, R, Yamazoe, M, Hiraga, S, Holland, B, Kohiyama, M and Malki, A (2000) Competition between the replication initiator DnaA and the sequestration factor SeqA for binding to the hemimethylated chromosomal origin of E. coli in vitro. Genes Cells 5:873-884 |
|---|---|
| Abstract |
Following replication initiation, the replication origin (oriC) in Escherichia coli enters a hemimethylated state at Dam methylation sites which are recognized by the SeqA protein. SeqA binds preferentially to hemimethylated GATC sequences of DNA in vitro. SeqA is essential for the synchronous initiation of chromosome replication from oriC copies in vivo. |
| Links | |
| Keywords |
AT Rich Sequence/physiology; Bacterial Outer Membrane Proteins; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacterial Proteins/pharmacology; Binding Sites/genetics; Binding, Competitive/genetics; DNA Methylation/drug effects; DNA Replication/drug effects; DNA Replication/genetics; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli; Escherichia coli Proteins; Origin Recognition Complex; Plasmids/genetics; Promoter Regions, Genetic/genetics; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Regulatory Sequences, Nucleic Acid/genetics; Replication Origin/genetics; Site-Specific DNA-Methyltransferase (Adenine-Specific)/antagonists & inhibitors; Transcription Factors; Viral Proteins/genetics |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.