PMID:11118303
| Citation |
Yamamura, E, Nunoshiba, T, Kawata, M and Yamamoto, K (2000) Characterization of spontaneous mutation in the oxyR strain of Escherichia coli. Biochem. Biophys. Res. Commun. 279:427-32 |
|---|---|
| Abstract |
Escherichia coli K-12 strain EY5, deficient in oxyR, was constructed to assess the role of oxyR and oxyR-regulated regulon in spontaneous mutagenesis. Mutagenesis was monitored by selecting two forward mutations of colicin B-sensitive to resistance and valine-sensitive to resistance, one base substitution mutation of rifampicin-sensitive to resistance and one reversion of argE3 his-4 to Arg(+) His(+). Deficiency of oxyR did not lead to the enhancement of spontaneous mutation frequencies of the four markers tested. By DNA sequence analysis, we determined 49 colicin B-resistant mutants derived from EY5 and found that 37% were base substitutions, 29% IS element insertions, 20% deletions, and 14% single base frameshifts. Among the base substitutions, G:C-->T:A transversions predominated followed by G:C-->A:T transitions and A:T-->T:A transversions. These spectra were essentially the same as those from oxyR(+) strains. The results indicate that oxyR and oxyR-regulated genes do not play a significant role in the defense against spontaneous mutagenesis. |
| Links |
PubMed Online version:10.1006/bbrc.2000.3961 |
| Keywords |
Bacterial Proteins/genetics; Base Sequence; DNA Transposable Elements; DNA-Binding Proteins; Escherichia coli/genetics; Escherichia coli Proteins; Frameshift Mutation; Genotype; Membrane Proteins/genetics; Mutation; Mutation, Missense; Regulon; Repressor Proteins/genetics; Sequence Deletion; Transcription Factors/genetics |
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