PMID:11118204
| Citation |
Francetic, O, Belin, D, Badaut, C and Pugsley, AP (2000) Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion. EMBO J. 19:6697-703 |
|---|---|
| Abstract |
Escherichia coli K-12, the most widely used laboratory bacterium, does not secrete proteins into the extracellular medium under standard growth conditions, despite possessing chromosomal genes encoding a putative type II secretion machinery (secreton). We show that in wild-type E.coli K-12, divergent transcription of the two operons in the main chromosomal gsp locus, encoding the majority of the secreton components, is silenced by the nucleoid-structuring protein H-NS. In mutants lacking H-NS, the secreton genes cloned on a moderate-copy-number plasmid are expressed and promote efficient secretion of the endogenous, co-regulated endochitinase ChiA. This is the first time that secretion of an endogenous extracellular protein has been demonstrated in E.coli K-12. |
| Links |
PubMed PMC305903 Online version:10.1093/emboj/19.24.6697 |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/secretion; Base Sequence; Chitinase; Chromosome Mapping; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Molecular Sequence Data; Operon; Plasmids; Transcription, Genetic |
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