PMID:11114917

Citation	Chalut, C, Charpentier, X, Remy, MH and Masson, JM (2001) Differential responses of Escherichia coli cells expressing cytoplasmic domain mutants of penicillin-binding protein 1b after impairment of penicillin-binding proteins 1a and 3. J. Bacteriol. 183:200-6
Abstract	Penicillin-binding protein 1b (PBP1b) is the major high-molecular-weight PBP in Escherichia coli. Although it is coded by a single gene, it is usually found as a mixture of three isoforms which vary with regard to the length of their N-terminal cytoplasmic tail. We show here that although the cytoplasmic tail seems to play no role in the dimerization of PBP1b, as was originally suspected, only the full-length protein is able to protect the cells against lysis when both PBP1a and PBP3 are inhibited by antibiotics. This suggests a specific role for the full-length PBP1b in the multienzyme peptidoglycan-synthesizing complex that cannot be fulfilled by either PBP1a or the shorter PBP1b proteins. Moreover, we have shown by alanine-stretch-scanning mutagenesis that (i) residues R(11) to G(13) are major determinants for correct translocation and folding of PBP1b and that (ii) the specific interactions involving the full-length PBP1b can be ascribed to the first six residues at the N-terminal end of the cytoplasmic domain. These results are discussed in terms of the interactions with other components of the murein-synthesizing complex.
Links	PubMed PMC94866 Online version:10.1128/JB.183.1.200-206.2001
Keywords	Amino Acid Sequence; Anti-Bacterial Agents/pharmacology; Aztreonam/pharmacology; Bacterial Proteins; Carrier Proteins; Cephaloridine/pharmacology; Cephalosporins/pharmacology; Cytoplasm/enzymology; Dimerization; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli Proteins; Hexosyltransferases/chemistry; Hexosyltransferases/genetics; Hexosyltransferases/metabolism; Isoenzymes/chemistry; Isoenzymes/genetics; Isoenzymes/metabolism; Molecular Sequence Data; Multienzyme Complexes/chemistry; Multienzyme Complexes/genetics; Multienzyme Complexes/metabolism; Muramoylpentapeptide Carboxypeptidase; Mutation; Penicillin-Binding Proteins; Peptidoglycan Glycosyltransferase; Peptidyl Transferases/chemistry; Peptidyl Transferases/genetics; Peptidyl Transferases/metabolism; Serine-Type D-Ala-D-Ala Carboxypeptidase
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

Useful Materials and Methods

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Annotations

<annotationlinks/>

EcoliWiki Links

Add links to pages that link here (e.g. gene, product, method pages)

References

See Help:References for how to manage references in EcoliWiki.