PMID:11111
| Citation |
Kittlick, PD, Neupert, G and Bolck, F (1976) Acid mucopolysaccharides in fibroblast cultures. 1. Influence of cell density, pH-value and lactate concentration on the MPS distribution pattern. Exp Pathol (Jena) 12:149-58 |
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| Abstract |
From cells and culture media of embryonic rat fibroblasts (1st subculture) the acid mucopolysaccharides were isolated and fractionated. The per cent calculation of the 6 fractions was based on the content of glucuronic acid. The cultures were maintained as follows: 0.5 X 10(6) to 3 X 10(6) cells were examined in Demeter flasks at pH 7.4 or 6.6; lactate concentration was enhanced to 100 mg%. The amount of each fraction was correlated with the cell density (linear regression). The pH-value and lactate concentration in connection with cell density proved to be important factors in the modification of the MPS distribution pattern. |
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| Keywords |
Animals; Cells, Cultured; Chondroitin Sulfates/metabolism; Dermatan Sulfate/metabolism; Embryo, Mammalian; Fibroblasts/metabolism; Glycosaminoglycans/analysis; Heparitin Sulfate/metabolism; Hyaluronic Acid/metabolism; Hydrogen-Ion Concentration; Lactates/analysis; Rats |
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