PMID:11094158
| Citation |
Okada, S, Okada, T, Aimi, T, Morinaga, T and Itoh, T (2000) HSP70 and ribosomal protein L2: novel 5S rRNA binding proteins in Escherichia coli. FEBS Lett. 485:153-6 |
|---|---|
| Abstract |
A Northwestern analysis of Escherichia coli total proteins with radiolabeled 5S rRNA identified two novel 5S rRNA interacting proteins, a 70 kDa and a 37 kDa protein, and three ribosomal proteins reported on already. The N-terminal sequencing of the 70 kDa protein separated by SDS-PAGE from the high-salt-washed fraction of crude ribosome led to the discovery of a polypeptide identical in its first 10 amino acid residues to E. coli heat shock protein 70. The N-terminal eight amino acid sequence of the 37 kDa protein extracted from the high-salt-washed ribosome is identical to that of the ribosomal protein L2. In addition, the interaction of these proteins with 5S rRNA has been confirmed with gel mobility shift assays. |
| Links | |
| Keywords |
Amino Acid Sequence; Electrophoresis, Polyacrylamide Gel; Escherichia coli/chemistry; HSP70 Heat-Shock Proteins/analysis; HSP70 Heat-Shock Proteins/chemistry; HSP70 Heat-Shock Proteins/metabolism; Peptide Fragments/chemistry; RNA, Ribosomal, 5S/metabolism; Ribosomal Proteins/analysis; Ribosomal Proteins/chemistry; Ribosomal Proteins/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.