PMID:11092872
| Citation |
Egan, SM, Pease, AJ, Lang, J, Li, X, Rao, V, Gillette, WK, Ruiz, R, Ramos, JL and Wolf, RE Jr (2000) Transcription activation by a variety of AraC/XylS family activators does not depend on the class II-specific activation determinant in the N-terminal domain of the RNA polymerase alpha subunit. J. Bacteriol. 182:7075-7 |
|---|---|
| Abstract |
The N-terminal domain of the RNA polymerase alpha subunit (alpha-NTD) was tested for a role in transcription activation by a variety of AraC/XylS family members. Based on substitutions at residues 162 to 165 and an extensive genetic screen we conclude that alpha-NTD is not an activation target for these activators. |
| Links | |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Cytarabine/metabolism; DNA-Binding Proteins; DNA-Directed RNA Polymerases/chemistry; DNA-Directed RNA Polymerases/genetics; DNA-Directed RNA Polymerases/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Mutation; Pseudomonas putida/genetics; Pseudomonas putida/metabolism; Sigma Factor/genetics; Sigma Factor/metabolism; Trans-Activators/genetics; Trans-Activators/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; Transcriptional Activation |
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