PMID:11092250
| Citation |
Trchounian, A and Kobayashi, H (2000) K+ uptake by fermenting Escherichia coli cells: pH dependent mode of the TrkA system operating. Biosci. Rep. 20:277-88 |
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| Abstract |
Escherichia coli accumulates K+ by means of multiple transport systems, of which TrkA is the most prominent at neutral and alkaline pH while Kup is major at acidic pH. In the present study, K+ uptake was observed with cells grown under fermentative conditions at an initial pH of 9.0 and 7.3 (the medium pH decreased to 8.4 and 6.8, respectively, during the mid-logarithmic growth phase), washed with distilled water and resuspended in a K+ containing medium at pH 7.5 in the presence of glucose. The kinetics for this K+ uptake and the amount of K+ accumulated by the wild type and mutants having a functional TrkA or Kup could confirm that K+ uptake by E. coli grown either at pH 9.0 or pH 7.3 occurs mainly through TrkA. The following results distinguish pH dependent mode of TrkA operating: (1) K+ uptake was inhibited by DCCD in cells grown either at pH 9.0 or pH 7.3, although the stoichiometry of K+ influx to DCCD-inhibited H+ efflux for bacteria grown at pH 9.0 varied with external K+ concentration, but remained constant for cells grown at pH 7.3; (2) K+ uptake was observed with an atpD mutant grown at pH 9.0 but not at pH 7.3; (3) The DCCD-inhibited H+ efflux was increased 8-fold less by 5 mM K+ added into a K+ free medium for bacteria grown at pH 9.0 than that for cells grown at pH 7.3; (4) the DCCD-inhibited ATPase activity of membrane vesicles from bacteria grown at pH 9.0 was reduced a little in the presence of 100 mM K+, but stimulated more than 2.4-fold at pH 7.3. |
| Links | |
| Keywords |
Carrier Proteins/drug effects; Carrier Proteins/metabolism; Dicyclohexylcarbodiimide/pharmacology; Escherichia coli/cytology; Escherichia coli/genetics; Escherichia coli/metabolism; Fermentation; Hydrogen-Ion Concentration; Membrane Proteins/drug effects; Membrane Proteins/metabolism; Membranes, Artificial; Mutation; Potassium/pharmacokinetics; Proton-Translocating ATPases/antagonists & inhibitors; Proton-Translocating ATPases/metabolism; Receptor, trkA |
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