PMID:11078889

Citation	Alcántara, C, Cervera, J and Rubio, V (2000) Carbamate kinase can replace in vivo carbamoyl phosphate synthetase. Implications for the evolution of carbamoyl phosphate biosynthesis. FEBS Lett. 484:261-4
Abstract	The exclusive involvement of carbamate kinase (CK) in fermentative ATP production and of carbamoyl phosphate synthetase (CPS) in the production of carbamoyl phosphate (CP) for pyrimidines and arginine biosynthesis was challenged by the finding of CK as the only activity synthesising CP in the archaea Pyrococcus furiosus and Pyrococcus abyssi. We now show that CK can replace CPS in vivo: transformation of Escherichia coli devoid of the CPS gene with plasmids encoding the CK from P. furiosus or from Enterococcus faecalis (which uses CK for making ATP) restores the ability of CPS-deficient E. coli to grow in the absence of arginine and uracil if ammonia and bicarbonate are present.
Links	PubMed
Keywords	Ammonia/metabolism; Arginine/metabolism; Bicarbonates/metabolism; Carbamoyl-Phosphate Synthase (Ammonia)/genetics; Carbamoyl-Phosphate Synthase (Ammonia)/metabolism; Enterococcus faecalis/enzymology; Enterococcus faecalis/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Kinetics; Phosphotransferases (Carboxyl Group Acceptor)/genetics; Phosphotransferases (Carboxyl Group Acceptor)/metabolism; Pyrococcus/enzymology; Pyrococcus/genetics; Transformation, Bacterial; Transformation, Genetic; Uracil/metabolism
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