PMID:11075344

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Citation

Burgess, RR, Arthur, TM and Pietz, BC (2000) Mapping protein-protein interaction domains using ordered fragment ladder far-western analysis of hexahistidine-tagged fusion proteins. Meth. Enzymol. 328:141-57

Abstract

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Keywords

Amino Acid Sequence; Blotting, Western/methods; Cloning, Molecular/methods; DNA-Directed RNA Polymerases/genetics; Electrophoresis, Polyacrylamide Gel/methods; Escherichia coli/enzymology; Escherichia coli/genetics; Hydroxylamine; Indicators and Reagents; Oligopeptides/chemistry; Protein Subunits; Recombinant Fusion Proteins/analysis; Recombinant Fusion Proteins/biosynthesis; Restriction Mapping; Thermolysin

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