PMID:11056219
| Citation |
Szilvay, AM, Stern, B, Blichenberg, A and Helland, DE (2000) Structural and functional similarities between HIV-1 reverse transcriptase and the Escherichia coli RNA polymerase beta' subunit. FEBS Lett. 484:43-7 |
|---|---|
| Abstract |
Four monoclonal antibodies (MAbs) recognizing HIV-1 reverse transcriptase (RT) were shown here to cross-react with the beta' subunit of Escherichia coli RNA polymerase (RNAP). The anti-RT MAbs bind to a peptide comprising residues 294-305 of the RT amino acid sequence. Computer analyses revealed sequence similarity between this peptide and two regions of the RNAP beta' subunit. MAb-binding studies using RT mutants suggested that the epitope is located to amino acids 652-663 of the beta' sequence. One of the MAbs which inhibited the polymerase activity of RT also mediated a dose dependent inhibition of the RNAP activity. |
| Links | |
| Keywords |
Amino Acid Sequence; Antibodies, Monoclonal/immunology; Antibodies, Monoclonal/pharmacology; Binding Sites; Blotting, Western; Catalysis/drug effects; Cross Reactions/immunology; DNA-Directed RNA Polymerases/antagonists & inhibitors; DNA-Directed RNA Polymerases/chemistry; DNA-Directed RNA Polymerases/immunology; DNA-Directed RNA Polymerases/metabolism; Dose-Response Relationship, Drug; Epitopes/chemistry; Epitopes/genetics; Epitopes/immunology; Escherichia coli/enzymology; HIV Reverse Transcriptase/chemistry; HIV Reverse Transcriptase/genetics; HIV Reverse Transcriptase/immunology; HIV Reverse Transcriptase/metabolism; HIV-1/enzymology; Kinetics; Molecular Sequence Data; Mutation/genetics; Peptide Fragments/chemistry; Peptide Fragments/genetics; Peptide Fragments/immunology; Protein Conformation; Protein Subunits; Structure-Activity Relationship |
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