PMID:11053405
| Citation |
Miller, S, Ness, LS, Wood, CM, Fox, BC and Booth, IR (2000) Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli. J. Bacteriol. 182:6536-40 |
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| Abstract |
A new subunit, YabF, for the KefC K(+) efflux system in Escherichia coli has been identified. The subunit is required for maximum activity of KefC. Deletion of yabF reduces KefC activity 10-fold, and supply of YabF in trans restores activity. IS2 and IS10R insertions in yabF can be isolated as suppressors of KefC activity consequent upon the V427A and D264A KefC mutations. |
| Links | |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Escherichia coli/chemistry; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Gene Deletion; Genes, Bacterial; Genes, Suppressor; Glutathione/metabolism; Mutation; Potassium Channels/genetics; Potassium Channels/metabolism |
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