PMID:11042274

Citation	Schneider, K, Dimroth, P and Bott, M (2000) Identification of triphosphoribosyl-dephospho-CoA as precursor of the citrate lyase prosthetic group. FEBS Lett. 483:165-8
Abstract	The gamma-subunit of citrate lyase (EC 4.1.3.6) contains the prosthetic group 2'-(5"-phosphoribosyl)-3'-dephospho-CoA and serves as an acyl carrier protein (ACP). We recently showed that in Escherichia coli the proteins CitG and CitX are essential for holo-ACP synthesis and provided evidence that CitG catalyzes the formation of a prosthetic group precursor from ATP and dephospho-CoA, which is subsequently attached via phosphodiester linkage to apo-ACP by CitX. Here we prove that CitG indeed catalyzes the conversion of ATP and dephospho-CoA to adenine and 2'-(5"-triphosphoribosyl)-3'-dephospho-CoA, the predicted precursor of the prosthetic group. Furthermore, this precursor was transferred by CitX to apo-ACP, yielding holo-ACP. Thus, our proposed mechanism for holo-ACP synthesis could be verified.
Links	PubMed
Keywords	ATP Citrate (pro-S)-Lyase/chemistry; ATP Citrate (pro-S)-Lyase/metabolism; Acyl Carrier Protein/metabolism; Adenosine Triphosphate/metabolism; Apoenzymes/metabolism; Chromatography, High Pressure Liquid; Coenzyme A/metabolism; Electrophoresis, Polyacrylamide Gel; Enzyme Precursors/metabolism; Escherichia coli/enzymology; Holoenzymes/metabolism; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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