PMID:11027315
| Citation |
Khodursky, AB, Peter, BJ, Cozzarelli, NR, Botstein, D, Brown, PO and Yanofsky, C (2000) DNA microarray analysis of gene expression in response to physiological and genetic changes that affect tryptophan metabolism in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 97:12170-5 |
|---|---|
| Abstract |
We investigated the global changes in mRNA abundance in Escherichia coli elicited by various perturbations of tryptophan metabolism. To do so we printed DNA microarrays containing 95% of all annotated E. coli ORFs. We determined the expression profile that is predominantly dictated by the activity of the tryptophan repressor. Only three operons, trp, mtr, and aroH, exhibited appreciable expression changes consistent with this profile. The quantitative changes we observed in mRNA levels for the five genes of the trp operon were consistent within a factor of 2, with expectations based on established Trp protein levels. Several operons known to be regulated by the TyrR protein, aroF-tyrA, aroL, aroP, and aroG, were down-regulated on addition of tryptophan. TyrR can be activated by any one of the three aromatic amino acids. Only one operon, tnaAB, was significantly activated by the presence of tryptophan in the medium. We uncovered a plethora of likely indirect effects of changes in tryptophan metabolism on intracellular mRNA pools, most prominent of which was the sensitivity of arginine biosynthetic operons to tryptophan starvation. |
| Links |
PubMed PMC17313 Online version:10.1073/pnas.220414297 |
| Keywords |
Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression Profiling; Multigene Family; Operon; RNA, Messenger/genetics; Repressor Proteins/genetics; Tryptophan/genetics; Tryptophan/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<protect><annotationlinks/></protect>
<protect>
| Accessions | SMD Category | SMD Subcategory | SMD status |
|---|---|---|---|
| edit table |
</protect>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.
