PMID:11027276
| Citation |
Cheng, C and Shuman, S (2000) Recombinogenic flap ligation pathway for intrinsic repair of topoisomerase IB-induced double-strand breaks. Mol. Cell. Biol. 20:8059-68 |
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| Abstract |
Topoisomerase IB catalyzes recombinogenic DNA strand transfer reactions in vitro and in vivo. Here we characterize a new pathway of topoisomerase-mediated DNA ligation in vitro (flap ligation) in which vaccinia virus topoisomerase bound to a blunt-end DNA joins the covalently held strand to a 5' resected end of a duplex DNA containing a 3' tail. The joining reaction occurs with high efficiency when the sequence of the 3' tail is complementary to that of the scissile strand immediately 5' of the cleavage site. A 6-nucleotide segment of complementarity suffices for efficient flap ligation. Invasion of the flap into the duplex apparently occurs while topoisomerase remains bound to DNA, thereby implying a conformational flexibility of the topoisomerase clamp around the DNA target site. The 3' flap acceptor DNA mimics a processed end in the double-strand-break-repair recombination pathway. Our findings suggest that topoisomerase-induced breaks may be rectified by flap ligation, with ensuing genomic deletions or translocations. |
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| Keywords |
Base Pairing; Base Sequence; DNA/genetics; DNA/metabolism; DNA Damage; DNA Repair; DNA Topoisomerases, Type I/metabolism; Gene Deletion; Kinetics; Models, Genetic; Molecular Sequence Data; Plasmids/metabolism; Recombination, Genetic; Time Factors; Translocation, Genetic; Vaccinia virus/enzymology; Vaccinia virus/genetics |
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