PMID:11009422
| Citation |
Levchenko, I, Seidel, M, Sauer, RT and Baker, TA (2000) A specificity-enhancing factor for the ClpXP degradation machine. Science 289:2354-6 |
|---|---|
| Abstract |
Events that stall bacterial protein synthesis activate the ssrA-tagging machinery, resulting in resumption of translation and addition of an 11-residue peptide to the carboxyl terminus of the nascent chain. This ssrA-encoded peptide tag marks the incomplete protein for degradation by the energy-dependent ClpXP protease. Here, a ribosome-associated protein, SspB, was found to bind specifically to ssrA-tagged proteins and to enhance recognition of these proteins by ClpXP. Cells with an sspB mutation are defective in degrading ssrA-tagged proteins, demonstrating that SspB is a specificity-enhancing factor for ClpXP that controls substrate choice. |
| Links | |
| Keywords |
Adenosine Triphosphatases/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Endopeptidase Clp; Escherichia coli/enzymology; Escherichia coli/metabolism; Escherichia coli Proteins; Green Fluorescent Proteins; Luminescent Proteins/metabolism; Mutation; Oligopeptides/chemistry; Oligopeptides/genetics; Oligopeptides/metabolism; Operon; Ribosomes/metabolism; Serine Endopeptidases/metabolism; Substrate Specificity |
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