PMID:10995223
| Citation |
Hermanns, U, Sebbel, P, Eggli, V and Glockshuber, R (2000) Characterization of FimC, a periplasmic assembly factor for biogenesis of type 1 pili in Escherichia coli. Biochemistry 39:11564-70 |
|---|---|
| Abstract |
Assembly of type 1 pili from Escherichia coli is mediated by FimC, a periplasmic chaperone (assembly factor) consisting of two immunoglobulin-like domains. FimC is assumed to recognize the individual pilus subunits in the periplasm mainly via their conserved C-terminal segments and to deliver the subunits to an assembly platform in the outer membrane. Here we present the first biochemical characterization of a periplasmic pilus chaperone and analyze the importance of the two chaperone domains for stability and function. Comparison of the isolated C-terminal domain with wild-type FimC revealed a strongly reduced thermodynamic stability, indicating strong interdomain interactions. The affinity of FimC toward a peptide corresponding to the 11 C-terminal residues of the type 1 pilus adhesin FimH is at least 1000-fold lower compared to binding of intact FimH, confirming that bacterial pilus chaperones, unlike other chaperones, specifically interact with folded pilus subunits. |
| Links | |
| Keywords |
Adhesins, Bacterial/metabolism; Adhesins, Escherichia coli; Amino Acid Sequence; Bacterial Outer Membrane Proteins/chemistry; Bacterial Outer Membrane Proteins/genetics; Bacterial Outer Membrane Proteins/isolation & purification; Bacterial Outer Membrane Proteins/metabolism; Bacterial Proteins; Circular Dichroism; Escherichia coli/chemistry; Escherichia coli/metabolism; Escherichia coli Proteins; Fimbriae Proteins; Fimbriae, Bacterial/chemistry; Fimbriae, Bacterial/metabolism; Molecular Chaperones/chemistry; Molecular Chaperones/genetics; Molecular Chaperones/isolation & purification; Molecular Chaperones/metabolism; Molecular Sequence Data; Peptide Fragments/biosynthesis; Peptide Fragments/chemistry; Peptide Fragments/genetics; Peptide Fragments/metabolism; Periplasm/chemistry; Periplasm/metabolism; Protein Binding; Protein Folding; Protein Structure, Tertiary/genetics; Recombinant Proteins/biosynthesis; Recombinant Proteins/chemistry; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Spectrometry, Fluorescence; Thermodynamics |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.