PMID:10981705
| Citation |
Stavropoulos, TA and Strathdee, CA (2000) Expression of the tetA(C) tetracycline efflux pump in Escherichia coli confers osmotic sensitivity. FEMS Microbiol. Lett. 190:147-50 |
|---|---|
| Abstract |
Expression of tetA(C) in Escherichia coli confers resistance to tetracycline as well as sensitivity to nickel and cadmium salts, lipophilic chelating agents, and aminoglycoside antibiotics. In this report we determine that high-level expression of tetA(C) also confers an osmotic sensitivity. The osmotic-sensitive phenotype is distinct from the tetracycline-resistant phenotype and can be localized to a domain contained within the first 98 amino acid residues of the TetA(C) polypeptide. |
| Links | |
| Keywords |
Antiporters/chemistry; Antiporters/genetics; Antiporters/metabolism; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Cell Membrane Permeability; Culture Media/chemistry; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/physiology; Nickel/pharmacology; Osmotic Pressure; Phenotype; Protein Structure, Tertiary; Tetracycline Resistance/genetics |
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