PMID:10966788
| Citation |
Trang, P, Kilani, A, Kim, J and Liu, F (2000) A ribozyme derived from the catalytic subunit of RNase P from Escherichia coli is highly effective in inhibiting replication of herpes simplex virus 1. J. Mol. Biol. 301:817-26 |
|---|---|
| Abstract |
A sequence-specific ribozyme (M1GS RNA) derived from the catalytic RNA subunit of RNase P from Escherichia coli was used to target the mRNA encoding human herpes simplex virus 1 (HSV-1) major transcription activator, ICP4. A reduction of more than 80% in the expression level of ICP4 and a reduction of about 1000-fold in viral growth were observed in cells that stably expressed the ribozyme. In contrast, a reduction of less than 10 % in ICP4 expression and viral growth was observed in cells that either did not express the ribozyme or produced a catalytically inactive ribozyme mutant. Thus, M1GS ribozyme is highly effective in inhibiting HSV-1 growth and can be used as a general gene-targeting agent for anti-HSV applications. |
| Links |
PubMed Online version:10.1006/jmbi.2000.4022 |
| Keywords |
Animals; Antiviral Agents/chemistry; Antiviral Agents/metabolism; Base Sequence; Catalytic Domain; Cell Line; Endoribonucleases/chemistry; Endoribonucleases/genetics; Endoribonucleases/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Viral; Gene Silencing; Genes, Viral/genetics; Herpesvirus 1, Human/growth & development; Herpesvirus 1, Human/physiology; Humans; Immediate-Early Proteins/antagonists & inhibitors; Immediate-Early Proteins/genetics; Immediate-Early Proteins/metabolism; RNA, Catalytic/chemistry; RNA, Catalytic/genetics; RNA, Catalytic/metabolism; RNA, Messenger/genetics; RNA, Messenger/metabolism; RNA, Viral/genetics; RNA, Viral/metabolism; Ribonuclease P; Substrate Specificity; Transcription Factors/antagonists & inhibitors; Transcription Factors/genetics; Transcription Factors/metabolism; Transfection; Virus Replication |
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