PMID:10956009
| Citation |
Sen, S, Karthikeyan, G and Rao, BJ (2000) RecA realigns suboptimally paired frames of DNA repeats through a process that requires ATP hydrolysis. Biochemistry 39:10196-206 |
|---|---|
| Abstract |
Microsatellite repeats such as mono-, di-, and trinucleotides are highly abundant and viable targets for homologous recombination in the genome. However, if recombination ensues in such repetitive regions, they are intrinsically prone to frame misalignments during pairing and might eventually give rise to genetic instabilities. Suboptimally paired frames lead to an abrogation of branch migration at the junctions of mixed sequences and repeats, due to a heterologous register. If so, can recombination machinery rectify such misalignments in order to avoid subsequent arrest in branch migration? We analyzed Escherichia coli RecA, the universal prototype of a recombinase, for its pairing abilities across repeats. We used a complementary pairing assay to test whether RecA can mediate realignments of stochastically paired suboptimal frames to a maximally aligned register. Here, we demonstrate that RecA-single stranded DNA filament indeed facilitates such a realignment, probably by sliding the paired strands across mono- and di- as well as trinucleotide repeats. These realignments apparently have no net directional bias. Such a putative "motor" function of RecA seems to be ATP hydrolysis-dependent. |
| Links | |
| Keywords |
Adenosine Triphosphate/metabolism; Base Pairing; Base Sequence; Binding Sites; DNA/metabolism; DNA-Binding Proteins/metabolism; Hydrolysis; Microsatellite Repeats; Models, Genetic; Molecular Sequence Data; Nucleic Acid Conformation; Oligodeoxyribonucleotides/metabolism; Protein Binding; Rec A Recombinases/metabolism; Recombination, Genetic |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.