PMID:10956007

Citation	Rice, KP, Chaput, JC, Cox, MM and Switzer, C (2000) RecA protein promotes strand exchange with DNA substrates containing isoguanine and 5-methyl isocytosine. Biochemistry 39:10177-88
Abstract	The Escherichia coli RecA protein pairs homologous DNA molecules and promotes DNA strand exchange in vitro. We have examined DNA strand exchange between a 70 nucleotide ssDNA fragment and a 40 bp duplex, in which all G and C residues (at 18 positions distributed throughout the 40 bp exchanged region) were replaced with the nonstandard nucleosides 2'-deoxyisoguanosine (iG) and 2'-deoxy-5-methylisocytidine (MiC), respectively. We demonstrate that the nonstandard oligonucleotides are substrates for the RecA protein, permitting DNA strand exchange in vitro at a rate and efficiency comparable to exchange with normal DNA substrates. This observation provides an expanded experimental basis for discussions of potential roles for iG and MiC in a genetic code. Experiments of this type also provide another avenue for exploring RecA-facilitated DNA pairing mechanisms.
Links	PubMed
Keywords	5-Methylcytosine/analogs & derivatives; Cytosine/analogs & derivatives; DNA/metabolism; DNA Replication; DNA, Single-Stranded/metabolism; DNA-Binding Proteins/metabolism; Guanine; Models, Chemical; Models, Molecular; Nucleic Acid Conformation; Oligodeoxyribonucleotides; Rec A Recombinases/metabolism; Recombination, Genetic; Substrate Specificity; Thermodynamics
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