PMID:10950190

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Citation

Blakeney, MD, Moulaei, T and DiChristina, TJ (2000) Fe(III) reduction activity and cytochrome content of Shewanella putrefaciens grown on ten compounds as sole terminal electron acceptor. Microbiol. Res. 155:87-94

Abstract

Shewanella putrefaciens was grown on a series of ten alternate compounds as sole terminal electron acceptor. Each cell type was analyzed for Fe(III) reduction activity, absorbance maxima in reduced-minus-oxidized difference spectra and heme-containing protein content. High-rate Fe(III) reduction activity, pronounced difference maxima at 521 and 551 nm and a predominant 29.3 kDa heme-containing protein expressed by cells grown on Fe(III), Mn(IV), U(VI), SO3(2-) and S2O3(2-), but not by cells grown on O2, NO3, NO2-, TMAO or fumarate. These results suggest that microbial Fe(III) reduction activity is enhanced by anaerobic growth on metals and sulfur compounds, yet is limited under all other terminal electron-accepting conditions.

Links

PubMed Online version:10.1016/S0944-5013(00)80042-7

Keywords

Cytochromes/analysis; Cytochromes/metabolism; Electrophoresis, Polyacrylamide Gel; Ferric Compounds/metabolism; Hemeproteins/analysis; Hemeproteins/metabolism; Oxidation-Reduction; Shewanella putrefaciens/growth & development; Shewanella putrefaciens/metabolism; Spectrophotometry

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