PMID:10947847

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Citation

Azam, TA, Hiraga, S and Ishihama, A (2000) Two types of localization of the DNA-binding proteins within the Escherichia coli nucleoid. Genes Cells 5:613-26

Abstract

The genome DNA of Escherichia coli is folded into the nucleosome-like structure, often called a nucleoid, by the binding of several DNA-binding proteins. We previously determined the specificity and affinity of DNA-binding for 12 species of the E. coli DNA-binding protein, and their intracellular concentrations at various growth phases. The intracellular localization of these proteins in E. coli could be predicted from these data, but no attempt has been made thus far to directly observe the intracellular distribution of the DNA-binding proteins.

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Keywords

Bacterial Proteins/isolation & purification; Carrier Proteins/isolation & purification; Cell Compartmentation; DNA-Binding Proteins/isolation & purification; DNA-Directed RNA Polymerases/isolation & purification; Escherichia coli/ultrastructure; Escherichia coli Proteins; Factor For Inversion Stimulation Protein; Fluorescent Antibody Technique, Indirect; Host Factor 1 Protein; Integration Host Factors; Repressor Proteins/isolation & purification; Ribosomal Proteins/isolation & purification; Ribosomes

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