PMID:10940054
| Citation |
Zhao, J and Winkler, ME (2000) Reduction of GC --> TA transversion mutation by overexpression of MutS in Escherichia coli K-12. J. Bacteriol. 182:5025-8 |
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| Abstract |
Overexpression of the MutS repair protein significantly decreased the rate of lacZ GC --> TA transversion mutation in stationary-phase and exponentially growing bacteria and in mutY and mutM mutants, which accumulate mismatches between 8-oxoguanine (8-oxoG) and adenine residues in DNA. Conversely, GC --> TA transversion increased in mutL or mutS mutants in stationary phase. In contrast, overexpression of MutS did not appreciably reduce lacZ AT --> CG transversion mutation in a mutT mutant. These results suggest that MutS-dependent repair can correct 8-oxoG:A mismatches in Escherichia coli cells but may not be able to compete with mutation fixation by MutY in mutT mutants. |
| Links | |
| Keywords |
Adenine; Adenosine Triphosphatases; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Pair Mismatch; Cystine; DNA Repair; DNA-Binding Proteins; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression; Genes, Bacterial; Guanine; MutS DNA Mismatch-Binding Protein; Mutation; Thymine |
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