PMID:1093180
| Citation |
Kopecko, DJ and Cohen, SN (1975) Site specific recA--independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci. Proc. Natl. Acad. Sci. U.S.A. 72:1373-7 |
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| Abstract |
A recA-independent recombinational event is described which results in insertion of an entire plasmid genome at a unique site of another plasmid, and coincident excision of a precisely defined DNA segment originally present at the point of the insertion. The resulting recombinant molecules subsequently can undergo site-specific translocation of their component segments or inversion of their original DNA sequence orientation. The events observed entail nonreciprocal exchange of genetic material, and involve a discrete nucleotide sequences that is duplicated in rotationally symmetrical reverse orientation on plasmid DNA (i.e., inverted repeat; palindrome). |
| Links | |
| Keywords |
Base Sequence; Centrifugation, Density Gradient; DNA, Bacterial/metabolism; Electrophoresis; Escherichia coli/metabolism; Escherichia coli/ultrastructure; Extrachromosomal Inheritance; Genes; Microscopy, Electron; Molecular Weight; Mutation; Plasmids; Recombination, Genetic; Transformation, Genetic; Translocation, Genetic |
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