PMID:10923791

Citation	Kato, A, Ohnishi, H, Yamamoto, K, Furuta, E, Tanabe, H and Utsumi, R (2000) Transcription of emrKY is regulated by the EvgA-EvgS two-component system in Escherichia coli K-12. Biosci. Biotechnol. Biochem. 64:1203-9
Abstract	Spontaneous mutations have been isolated in Escherichia coli that result in the constitutive expression of an emrKY promoter. These mutations were found to be single-nucleotide substitutions within the linker region of the sensor protein EvgS, which is part of a two-component regulatory system along with EvgA. In the linker mutants (evgSI and evgS4), emrKY expression became constitutive and MIC against sodium deoxycholate was 20 mg/ml, eight-fold higher than in the wild type. Furthermore, the start site of transcription from the promoter of emrKY was identified; EvgA was shown to bind at the -52 to -84 region by the footprinting experiment.
Links	PubMed
Keywords	Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Binding Sites/genetics; DNA Primers/genetics; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; Drug Resistance, Microbial/genetics; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Genes, Bacterial; Molecular Sequence Data; Mutation; Promoter Regions, Genetic; Transcription Factors/genetics; Transcription Factors/metabolism; Transcription, Genetic
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