PMID:10921881
| Citation |
Jia, Y, McAdams, SA, Bryan, GT, Hershey, HP and Valent, B (2000) Direct interaction of resistance gene and avirulence gene products confers rice blast resistance. EMBO J. 19:4004-14 |
|---|---|
| Abstract |
Rice expressing the Pi-ta gene is resistant to strains of the rice blast fungus, Magnaporthe grisea, expressing AVR-Pita in a gene-for-gene relationship. Pi-ta encodes a putative cytoplasmic receptor with a centrally localized nucleotide-binding site and leucine-rich domain (LRD) at the C-terminus. AVR-Pita is predicted to encode a metalloprotease with an N-terminal secretory signal and pro-protein sequences. AVR-Pita(176) lacks the secretory and pro-protein sequences. We report here that transient expression of AVR-Pita(176) inside plant cells results in a Pi-ta-dependent resistance response. AVR-Pita(176) protein is shown to bind specifically to the LRD of the Pi-ta protein, both in the yeast two-hybrid system and in an in vitro binding assay. Single amino acid substitutions in the Pi-ta LRD or in the AVR-Pita(176) protease motif that result in loss of resistance in the plant also disrupt the physical interaction, both in yeast and in vitro. These data suggest that the AVR-Pita(176) protein binds directly to the Pi-ta LRD region inside the plant cell to initiate a Pi-ta-mediated defense response. |
| Links |
PubMed PMC306585 Online version:10.1093/emboj/19.15.4004 |
| Keywords |
Binding Sites; Biolistics; Gene Transfer Techniques; Genes, Fungal; Genes, Plant; Magnaporthe/genetics; Magnaporthe/pathogenicity; Metalloendopeptidases/genetics; Metalloendopeptidases/metabolism; Oryza sativa/genetics; Plant Diseases/genetics; Plant Leaves/microbiology; Plant Proteins; Protein Binding; Receptors, Cytoplasmic and Nuclear/genetics; Receptors, Cytoplasmic and Nuclear/metabolism; Two-Hybrid System Techniques |
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