PMID:1091626
| Citation |
Marinus, MG, Morris, NR, Söll, D and Kwong, TC (1975) Isolation and partial characterization of three Escherichia coli mutants with altered transfer ribonucleic acid methylases. J. Bacteriol. 122:257-65 |
|---|---|
| Abstract |
Seven transfer ribonucleic acid (tRNA) methylase mutants were isolated from Escherichia coli K-12 by examining the ability of RNA prepared from clones of unselected mutagenized cells to accept methyl groups from S-adenosylmethionine catalyzed by crude enzymes from wild-type cells. Five of the mutants had an altered uracil-tRNA methylase; consequently their tRNA's lacked ribothymidine. One mutant had tRNA deficient in 7-methylguanosine, and one mutant contained tRNA lacking 2-thio-5-methylaminomethyluridine. The genetic loci of the three tRNA methylase mutants were distributed over the E. coli genome. The mutant strain deficient in 7-methylguanosine biosynthesis showed a reduced efficiency in the suppression of amber mutations carried by T4 or lambda phages. |
| Links | |
| Keywords |
Chromatography, Affinity; Chromatography, Thin Layer; Chromosome Mapping; Electrophoresis; Escherichia coli/enzymology; Escherichia coli/isolation & purification; Genes; Guanosine/analogs & derivatives; Guanosine/biosynthesis; Methyltransferases/metabolism; Mutagens; Mutation; Nitrosoguanidines; RNA, Bacterial/metabolism; RNA, Ribosomal/metabolism; RNA, Transfer/metabolism; Recombination, Genetic; S-Adenosylmethionine/metabolism; Thymidine/analogs & derivatives; Thymidine/biosynthesis; Uridine/analogs & derivatives; Uridine/biosynthesis |
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