PMID:10913108
| Citation |
Ko, M and Park, C (2000) H-NS-Dependent regulation of flagellar synthesis is mediated by a LysR family protein. J. Bacteriol. 182:4670-2 |
|---|---|
| Abstract |
H-NS regulates the flagellar master operon (flhDC) and thus is necessary for flagellation of Escherichia coli. However, the molecular mechanism of its regulation has remained unknown. Genetic screening of a transposon insertion abolishing the H-NS effect revealed a previously unidentified gene, named hdfR, encoding a LysR family protein. Binding of purified HdfR to the flhDC promoter was demonstrated by a DNA mobility shift assay, indicating that HdfR is a transcriptional regulator for the flagellar master operon. Furthermore, the expression of the hdfR gene was shown to be negatively regulated by H-NS. |
| Links | |
| Keywords |
Bacterial Proteins/metabolism; Chromosomes, Bacterial/genetics; DNA-Binding Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Flagella/genetics; Gene Expression Regulation, Bacterial; Mutagenesis, Insertional; Operon; Repressor Proteins/metabolism; Restriction Mapping; Transcription Factors/metabolism |
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