PMID:10881193
| Citation |
Altieri, AS, Mazzulla, MJ, Horita, DA, Coats, RH, Wingfield, PT, Das, A, Court, DL and Byrd, RA (2000) The structure of the transcriptional antiterminator NusB from Escherichia coli. Nat. Struct. Biol. 7:470-4 |
|---|---|
| Abstract |
We have determined the solution structure of NusB, a transcription antitermination protein from Escherichia coli. The structure reveals a novel, all alpha-helical protein fold. NusB mutations that cause a loss of function (NusB5) or alter specificity for RNA targets (NusB101) are localized to surface residues and likely affect RNA-protein or protein-protein interactions. Residues that are highly conserved among homologs stabilize the protein core. The solution structure of E. coli NusB presented here resembles that of Mycobacterium tuberculosis NusB determined by X-ray diffraction, but differs substantially from a solution structure of E. coli NusB reported earlier. |
| Links |
PubMed Online version:10.1038/75869 |
| Keywords |
Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Binding Sites; Conserved Sequence; Crystallography, X-Ray; Escherichia coli/chemistry; Escherichia coli/genetics; Escherichia coli Proteins; Models, Molecular; Molecular Sequence Data; Mutation/genetics; Mycobacterium tuberculosis/chemistry; Nuclear Magnetic Resonance, Biomolecular; Protein Binding; Protein Structure, Secondary; RNA/genetics; RNA/metabolism; RNA-Binding Proteins/chemistry; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism; Sequence Alignment; Solutions; Transcription Factors/chemistry; Transcription Factors/genetics; Transcription Factors/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.