PMID:10873450
| Citation |
Tran, VK, Oropeza, R and Kenney, LJ (2000) A single amino acid substitution in the C terminus of OmpR alters DNA recognition and phosphorylation. J. Mol. Biol. 299:1257-70 |
|---|---|
| Abstract |
In bacteria and lower eukaryotes, adaptation to changes in the environment is often mediated by two-component regulatory systems. Such systems provide the basis for chemotaxis, nitrogen and phosphate regulation and adaptation to osmotic stress, for example. In Escherichia coli, the sensor kinase EnvZ detects a change in the osmotic environment and phosphorylates the response regulator OmpR. Phospho-OmpR binds to the regulatory regions of the porin genes ompF and ompC, and alters their expression. Recent evidence suggests that OmpR functions as a global regulator, regulating additional genes besides the porin genes. In this study, we have characterized a previously isolated OmpR2 mutant (V203M) that constitutively activates ompF and fails to express ompC. Because the substitution was located in the C-terminal DNA-binding domain, it had been assumed that the substitution would not affect phosphorylation of the N-terminal domain of OmpR. Our results indicate that this substitution completely eliminates phosphorylation by a small phosphate donor, acetyl phosphate, but not phosphorylation by the kinase EnvZ. The mutant OmpR has altered dephosphorylation kinetics and altered binding affinities to both ompF and ompC sites compared to the wild-type. Thus, a single amino acid substitution in the C-terminal DNA-binding domain has dramatic effects on the N-terminal phosphorylation domain. Most strikingly, we have identified a single base change in the OmpR binding site of ompC that restores high-affinity binding activity by the mutant. We interpret our results in the context of a model for porin gene expression. |
| Links |
PubMed Online version:10.1006/jmbi.2000.3809 |
| Keywords |
Amino Acid Substitution/genetics; Bacterial Outer Membrane Proteins/chemistry; Bacterial Outer Membrane Proteins/genetics; Bacterial Outer Membrane Proteins/isolation & purification; Bacterial Outer Membrane Proteins/metabolism; Base Sequence; Chromatography, High Pressure Liquid; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; Escherichia coli/chemistry; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Models, Genetic; Molecular Weight; Multienzyme Complexes; Mutation/genetics; Phosphates/metabolism; Phosphoproteins/chemistry; Phosphoproteins/genetics; Phosphoproteins/isolation & purification; Phosphoproteins/metabolism; Phosphorylation; Porins/genetics; Protein Binding; Response Elements/genetics; Sequence Alignment; Signal Transduction; Substrate Specificity; Thermodynamics; beta-Galactosidase/genetics; beta-Galactosidase/metabolism |
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