PMID:10872319
| Citation |
von Wilcken-Bergmann, B, Koenen, M, Griesser, HW and Müller-Hill, B (1983) 72 residues of gal repressor fused to beta-galactosidase repress the gal operon of E. coli. EMBO J. 2:1271-4 |
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| Abstract |
An active gene has been constructed which produces a chimera consisting of the N-terminal domain of the gal repressor and all but the first five residues of beta-galactosidase. Seventy two residues of gal repressor fused to beta-galactosidase as tetrameric core are sufficient to repress the gal operon in vivo and to bind to the gal operator in vitro. |
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| Keywords |
Amino Acid Sequence; Artificial Gene Fusion; Base Sequence; DNA, Bacterial; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Molecular Sequence Data; Operator Regions, Genetic; Operon; Repressor Proteins/genetics; beta-Galactosidase/genetics |
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