PMID:10869097
| Citation |
Boyle, DS, Grant, D, Draper, GC and Donachie, WD (2000) All major regions of FtsK are required for resolution of chromosome dimers. J. Bacteriol. 182:4124-7 |
|---|---|
| Abstract |
Resolution of chromosome dimers, by site-specific recombination between dif sites, is carried out in Escherichia coli by XerCD recombinase in association with the FtsK protein. We show here that a variety of altered FtsK polypeptides, consisting of the N-terminal (cell division) domain alone or with deletions in the proline-glutamine-rich part of the protein, or polypeptides consisting of the C-terminal domain alone are all unable to carry out dif recombination. Alteration of the putative nucleotide-binding site also abolishes the ability of FtsK to carry out recombination between dif sites. |
| Links | |
| Keywords |
Chromosomes, Bacterial; Escherichia coli/genetics; Escherichia coli Proteins; Membrane Proteins/metabolism; Recombination, Genetic; Sequence Deletion |
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