PMID:10860746
| Citation |
Sergiev, PV, Bogdanov, AA, Dahlberg, AE and Dontsova, O (2000) Mutations at position A960 of E. coli 23 S ribosomal RNA influence the structure of 5 S ribosomal RNA and the peptidyltransferase region of 23 S ribosomal RNA. J. Mol. Biol. 299:379-89 |
|---|---|
| Abstract |
The proximity of loop D of 5 S rRNA to two regions of 23 S rRNA, domain II involved in translocation and domain V involved in peptide bond formation, is known from previous cross-linking experiments. Here, we have used site-directed mutagenesis and chemical probing to further define these contacts and possible sites of communication between 5 S and 23 S rRNA. Three different mutants were constructed at position A960, a highly conserved nucleotide in domain II previously crosslinked to 5 S rRNA, and the mutant rRNAs were expressed from plasmids as homogeneous populations of ribosomes in Escherichia coli deficient in all seven chromosomal copies of the rRNA operon. Mutations A960U, A960G and, particularly, A960C caused structural rearrangements in the loop D of 5 S rRNA and in the peptidyltransferase region of domain V, as well as in the 960 loop itself. These observations support the proposal that loop D of 5 S rRNA participates in signal transmission between the ribosome centers responsible for peptide bond formation and translocation. |
| Links |
PubMed Online version:10.1006/jmbi.2000.3739 |
| Keywords |
Aldehydes/metabolism; Base Sequence; Binding Sites; CME-Carbodiimide/analogs & derivatives; CME-Carbodiimide/metabolism; Escherichia coli/genetics; Escherichia coli/growth & development; GTP Phosphohydrolases/chemistry; GTP Phosphohydrolases/genetics; GTP Phosphohydrolases/metabolism; Genes, Bacterial/genetics; Molecular Sequence Data; Mutation/genetics; Nucleic Acid Conformation; Peptidyl Transferases/chemistry; Peptidyl Transferases/genetics; Peptidyl Transferases/metabolism; Phenotype; Protein Biosynthesis; RNA, Bacterial/chemistry; RNA, Bacterial/genetics; RNA, Bacterial/metabolism; RNA, Ribosomal, 23S/chemistry; RNA, Ribosomal, 23S/genetics; RNA, Ribosomal, 23S/metabolism; RNA, Ribosomal, 5S/chemistry; RNA, Ribosomal, 5S/genetics; RNA, Ribosomal, 5S/metabolism; RNA, Transfer/chemistry; RNA, Transfer/genetics; RNA, Transfer/metabolism; RNA-Binding Proteins/chemistry; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism; Ribosomes/chemistry; Ribosomes/genetics; Ribosomes/metabolism; Structure-Activity Relationship; Sulfuric Acid Esters/metabolism; rRNA Operon/genetics |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.