PMID:10856643
| Citation |
Hutchings, MI and Drabble, WT (2000) Regulation of the divergent guaBA and xseA promoters of Escherichia coli by the cyclic AMP receptor protein. FEMS Microbiol. Lett. 187:115-22 |
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| Abstract |
The gua promoter (guaP) of Escherichia coli resembles those for ribosomal RNA (rrn) operons and lies in a close back-to-back arrangement with the promoter for xseA (xseP). Transcription from guaP is subject to stringent control and growth-rate-dependent regulation, and to repression by DnaA and PurR. In addition, transcription from guaP is regulated by the cyclic AMP receptor protein (CRP). Plasmid-borne promoter fusions to the receptor gene for chloramphenicol acetyl transferase were used to assess the role of CRP in controlling transcription from guaP and xseP following a downshift of cultures from rich into minimal medium. CRP is required to activate guaBA transcription and repress xseA transcription following downshift. Bandshift assays with a DNA fragment carrying the divergent promoters revealed specific binding of CRP. We propose that CRP, binding to a near-consensus site centred at -117.5, activates transcription from guaP and obstructs transcription from the xseA promoter. |
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| Keywords |
Base Sequence; Culture Media; Cyclic AMP Receptor Protein/metabolism; Cyclic AMP Receptor Protein/pharmacology; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Exodeoxyribonucleases/genetics; Exodeoxyribonucleases/metabolism; Gene Expression Regulation, Bacterial; Guanine Nucleotides/biosynthesis; Molecular Sequence Data; Operon; Plasmids; Promoter Regions, Genetic/genetics; Transcription, Genetic |
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