PMID:10850814

Citation	Blance, SJ, Williams, NL, Preston, ZA, Bishara, J, Smyth, MS and Maxwell, A (2000) Temperature-sensitive suppressor mutations of the Escherichia coli DNA gyrase B protein. Protein Sci. 9:1035-7
Abstract	Escherichia coli strain LE316 contains a mutation in gyrB that results in the substitution of Val164 to Gly and confers both chlorobiocin resistance and temperature sensitivity. Selection for suppressors of the ts phenotype yielded second-site mutations in GyrB at His38 and Thr157. The properties of proteins bearing these mutations have been characterized, and a mechanism of suppression is proposed based upon structural considerations.
Links	PubMed PMC2144630 Online version:10.1110/ps.9.5.1035
Keywords	Antibiotics, Antineoplastic/pharmacology; Coumarins/chemistry; Crystallography, X-Ray; DNA Gyrase; DNA Topoisomerases, Type II/chemistry; DNA Topoisomerases, Type II/genetics; Drug Resistance, Neoplasm; Enzyme Inhibitors/pharmacology; Escherichia coli/enzymology; Escherichia coli/genetics; Glycine/chemistry; Models, Chemical; Models, Molecular; Mutagenesis, Site-Directed; Novobiocin/analogs & derivatives; Novobiocin/pharmacology; Phenotype; Protein Conformation; Suppression, Genetic; Temperature; Thermodynamics; Valine/chemistry
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