PMID:10844678

Citation	Blinkova, A, Ginés-Candelaria, E, Ross, JD and Walker, JR (2000) Suppression of a DnaX temperature-sensitive polymerization defect by mutation in the initiation gene, dnaA, requires functional oriC. Mol. Microbiol. 36:913-25
Abstract	Temperature sensitivity of DNA polymerization and growth, resulting from mutation of the tau and gamma subunits of Escherichia coli DNA polymerase III, are suppressed by Cs,Sx mutations of the initiator gene, dnaA. These mutations simultaneously cause defective initiation at 20 degrees C. Efficient suppression, defined as restoration of normal growth rate at 39 degrees C to essentially all the cells, depends on functional oriC. Increasing DnaA activity in a strain capable of suppression, by introducing a copy of the wild-type allele, increasing the suppressor gene dosage or introducing a seqA mutation, reversed the suppression. This suggests that the suppression mechanism depends on reduced activity of DnaACs, Sx. Models that assume that suppression results from an initiation defect or from DnaACs,Sx interaction with polymerization proteins during nascent strand synthesis are proposed.
Links	PubMed
Keywords	Bacterial Proteins/genetics; DNA Polymerase III/genetics; DNA Replication; DNA-Binding Proteins/genetics; Escherichia coli/genetics; Escherichia coli/growth & development; Genes, Bacterial; Mutagenesis; Polymers; Replication Origin; Suppression, Genetic; Temperature
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.