PMID:10841758
| Citation |
Madore, E, Lipman, RS, Hou, YM and Lapointe, J (2000) Evidence for unfolding of the single-stranded GCCA 3'-End of a tRNA on its aminoacyl-tRNA synthetase from a stacked helical to a foldback conformation. Biochemistry 39:6791-8 |
|---|---|
| Abstract |
The conformation of a tRNA in its initial contact with its cognate aminoacyl-tRNA synthetase was investigated with the Escherichia coli glutamyl-tRNA synthetase-tRNA(Glu) complex. Covalent complexes between the periodate-oxidized tRNA(Glu) and its synthetase were obtained. These complexes are specific since none were formed with any other oxidized E. coli tRNA. The three major residues cross-linked to the 3'-terminal adenosine of oxidized tRNA(Glu) are Lys115, Arg209, and Arg48. Modeling of the tRNA(Glu)-glutamyl-tRNA synthetase based on the known crystal structures of Thermus thermophilus GluRS and of the E. coli tRNA(Gln)-glutaminyl-tRNA synthetase complex shows that these three residues are located in the pocket that binds the acceptor stem, and that Lys115, located in a 26 residue loop closed by coordination to a zinc atom in the tRNA acceptor stem-binding domain, is the first contact point of the 3'-terminal adenosine of tRNA(Glu). In our model, we assume that the 3'-terminal GCCA single-stranded segment of tRNA(Glu) is helical and extends the stacking of the acceptor stem. This assumption is supported by the fact that the 3' CCA sequence of tRNA(Glu) is not readily circularized in the presence of T4 RNA ligase under conditions where several other tRNAs are circularized. The two other cross-linked sites are interpreted as the contact sites of the 3'-terminal ribose on the enzyme during the unfolding and movement of the 3'-terminal GCCA segment to position the acceptor ribose in the catalytic site for aminoacylation. |
| Links | |
| Keywords |
Amino Acid Sequence; Amino Acyl-tRNA Synthetases/chemistry; Binding Sites; Electrophoresis, Gel, Two-Dimensional; Escherichia coli/chemistry; Escherichia coli/enzymology; Glutamate-tRNA Ligase/chemistry; Models, Molecular; Molecular Sequence Data; Nucleic Acid Conformation; Peptide Fragments/analysis; Periodic Acid/chemistry; RNA Ligase (ATP)/metabolism; RNA, Transfer, Gln/chemistry; RNA, Transfer, Glu/chemistry; Sequence Alignment; Thermus thermophilus/chemistry; Thermus thermophilus/enzymology; Trypsin |
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