PMID:10838053
| Citation |
Fillingame, RH, Jiang, W, Dmitriev, OY and Jones, PC (2000) Structural interpretations of F(0) rotary function in the Escherichia coli F(1)F(0) ATP synthase. Biochim. Biophys. Acta 1458:387-403 |
|---|---|
| Abstract |
F(1)F(0) ATP synthases are known to synthesize ATP by rotary catalysis in the F(1) sector of the enzyme. Proton translocation through the F(0) membrane sector is now proposed to drive rotation of an oligomer of c subunits, which in turn drives rotation of subunit gamma in F(1). The primary emphasis of this review will be on recent work from our laboratory on the structural organization of F(0), which proves to be consistent with the concept of a c(12) oligomeric rotor. From the NMR structure of subunit c and cross-linking studies, we can now suggest a detailed model for the organization of the c(12) oligomer in F(0) and some of the transmembrane interactions with subunits a and b. The structural model indicates that the H(+)-carrying carboxyl of subunit c is located between subunits of the c(12) oligomer and that two c subunits pack in a front-to-back manner to form the proton (cation) binding site. The proton carrying Asp61 side chain is occluded between subunits and access to it, for protonation and deprotonation via alternate entrance and exit half-channels, requires a swiveled opening of the packed c subunits and stepwise association with different transmembrane helices of subunit a. We suggest how some of the structural information can be incorporated into models of rotary movement of the c(12) oligomer during coupled synthesis of ATP in the F(1) portion of the molecule. |
| Links | |
| Keywords |
Adenosine Triphosphate/biosynthesis; Bacterial Proteins/chemistry; Binding Sites; Escherichia coli/enzymology; Magnetic Resonance Spectroscopy; Membrane Proteins/chemistry; Models, Molecular; Molecular Motor Proteins/chemistry; Propionibacterium/enzymology; Protein Conformation; Protein Folding; Proton-Translocating ATPases/chemistry |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.