PMID:10833520
| Citation |
Escolar, L, Pérez-Martín, J and de Lorenzo, V (2000) Evidence of an unusually long operator for the fur repressor in the aerobactin promoter of Escherichia coli. J. Biol. Chem. 275:24709-14 |
|---|---|
| Abstract |
Production of the siderophore aerobactin in Escherichia coli is transcriptionally metalloregulated through the iron-dependent binding of the Fur (ferric uptake regulator) to a large region (>100 base pairs) within the cognate promoter in the pColV-K30 plasmid. We show in this article that such an unusually long operator results from the specific addition of degenerate repeats 5'-NAT(A/T)AT-3' and not from a fortuitous occupation of the DNA adjacent to the primary binding sites by an excess of the repressor. Furthermore, the protection pattern revealed by DNase I and hydroxyl radical footprinting reflected a side-by-side oligomerization of the protein along an extended DNA stretch. This type of DNA-protein interactions is more like those observed in some eukaryotic factors and nucleoid-associated proteins than typical of specific prokaryotic regulators. |
| Links |
PubMed Online version:10.1074/jbc.M002839200 |
| Keywords |
5' Untranslated Regions/genetics; Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Base Sequence; Binding Sites; DNA Footprinting; Deoxyribonuclease I; Escherichia coli/genetics; Frameshift Mutation; Genetic Variation; Hydroxamic Acids/metabolism; Hydroxyl Radical; Iron/metabolism; Molecular Sequence Data; Operator Regions, Genetic; Promoter Regions, Genetic; Repressor Proteins/isolation & purification; Repressor Proteins/metabolism; Siderophores/metabolism |
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