PMID:10829077

Citation	McKenzie, GJ, Harris, RS, Lee, PL and Rosenberg, SM (2000) The SOS response regulates adaptive mutation. Proc. Natl. Acad. Sci. U.S.A. 97:6646-51
Abstract	Upon starvation some Escherichia coli cells undergo a transient, genome-wide hypermutation (called adaptive mutation) that is recombination-dependent and appears to be a response to a stressful environment. Adaptive mutation may reflect an inducible mechanism that generates genetic variability in times of stress. Previously, however, the regulatory components and signal transduction pathways controlling adaptive mutation were unknown. Here we show that adaptive mutation is regulated by the SOS response, a complex, graded response to DNA damage that includes induction of gene products blocking cell division and promoting mutation, recombination, and DNA repair. We find that SOS-induced levels of proteins other than RecA are needed for adaptive mutation. We report a requirement of RecF for efficient adaptive mutation and provide evidence that the role of RecF in mutation is to allow SOS induction. We also report the discovery of an SOS-controlled inhibitor of adaptive mutation, PsiB. These results indicate that adaptive mutation is a tightly regulated response, controlled both positively and negatively by the SOS system.
Links	PubMed PMC18688 Online version:10.1073/pnas.120161797
Keywords	Adaptation, Physiological; Bacterial Proteins/physiology; DNA Repair; DNA-Binding Proteins/physiology; Escherichia coli Proteins; Mutation; Regulon; SOS Response (Genetics); Serine Endopeptidases/physiology; Signal Transduction
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

Useful Materials and Methods

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Annotations

<annotationlinks/>

EcoliWiki Links

Add links to pages that link here (e.g. gene, product, method pages)

References

See Help:References for how to manage references in EcoliWiki.