PMID:10829063

Citation	Levin-Zaidman, S, Frenkiel-Krispin, D, Shimoni, E, Sabanay, I, Wolf, SG and Minsky, A (2000) Ordered intracellular RecA-DNA assemblies: a potential site of in vivo RecA-mediated activities. Proc. Natl. Acad. Sci. U.S.A. 97:6791-6
Abstract	The inducible SOS response increases the ability of bacteria to cope with DNA damage through various DNA repair processes in which the RecA protein plays a central role. Here we present the first study of the morphological aspects that accompany the SOS response in Escherichia coli. We find that induction of the SOS system in wild-type bacteria results in a fast and massive intracellular coaggregation of RecA and DNA into a lateral macroscopic assembly. The coaggregates comprise substantial portions of both the cellular RecA and the DNA complement. The structural features of the coaggregates and their relation to in vitro RecA-DNA networks, as well as morphological studies of strains carrying RecA mutants, are all consistent with the possibility that the intracellular assemblies represent a functional entity in which RecA-mediated DNA repair and protection activities occur.
Links	PubMed PMC18741 Online version:10.1073/pnas.090532397
Keywords	DNA Damage; DNA Repair; Escherichia coli/genetics; Rec A Recombinases/chemistry; SOS Response (Genetics)
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