PMID:10818234

Citation	Rodríguez-Concepción, M, Campos, N, María Lois, L, Maldonado, C, Hoeffler, JF, Grosdemange-Billiard, C, Rohmer, M and Boronat, A (2000) Genetic evidence of branching in the isoprenoid pathway for the production of isopentenyl diphosphate and dimethylallyl diphosphate in Escherichia coli. FEBS Lett. 473:328-32
Abstract	An alternative mevalonate-independent pathway for isoprenoid biosynthesis has been recently discovered in eubacteria (including Escherichia coli) and plant plastids, although it is not fully elucidated yet. In this work, E. coli cells were engineered to utilize exogenously provided mevalonate and used to demonstrate by a genetic approach that branching of the endogenous pathway results in separate synthesis of the isoprenoid building units isopentenyl diphosphate (IPP) and its isomer dimethylallyl diphosphate (DMAPP). In addition, the IPP isomerase encoded by the idi gene was shown to be functional in vivo and to represent the only possibility for interconverting IPP and DMAPP in this bacterium.
Links	PubMed
Keywords	Arabinose/metabolism; Carbon-Carbon Double Bond Isomerases/genetics; Carbon-Carbon Double Bond Isomerases/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Hemiterpenes; Mevalonic Acid/metabolism; Mutagenesis, Insertional; Organophosphorus Compounds/metabolism; Polymerase Chain Reaction
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