PMID:10802061
| Citation |
Binet, MR and Poole, RK (2000) Cd(II), Pb(II) and Zn(II) ions regulate expression of the metal-transporting P-type ATPase ZntA in Escherichia coli. FEBS Lett. 473:67-70 |
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| Abstract |
ZntA is a cation-translocating ATPase which exports from Escherichia coli Cd(II) and Pb(II), as well as Zn(II). The metal-dependent ATP hydrolysis activity of purified ZntA was recently characterised and showed a specificity for Cd(II), Pb(II) and Zn(II). zntA expression has been reported to be up-regulated primarily by Zn(II), mediated by the regulatory protein ZntR, belonging to the MerR transcriptional regulator family. In contrast to previous claims, we now show, using a Phi(zntA-lacZ) monolysogen, that Cd(II) is the most effective inducer of zntA, which is also induced significantly by Pb(II). The Cd(II)- and Pb(II)-dependent transcriptional up-regulation of zntA is also mediated by ZntR. |
| Links | |
| Keywords |
Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Adenosine Triphosphate/metabolism; Bacterial Proteins; Cadmium/metabolism; Cadmium/pharmacology; Cations, Divalent/metabolism; Cations, Divalent/pharmacology; Drug Resistance, Microbial; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins; Gene Dosage; Gene Expression Regulation, Bacterial/drug effects; Genes, Bacterial/genetics; Hydrolysis/drug effects; Lead/metabolism; Lead/pharmacology; Microbial Sensitivity Tests; Mutation/genetics; Operon/genetics; Promoter Regions, Genetic/genetics; Substrate Specificity; Transcription Factors/genetics; Transcription Factors/metabolism; Up-Regulation/drug effects; Zinc/metabolism; Zinc/pharmacology |
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