PMID:10792731
| Citation |
O'Gara, JP and Dorman, CJ (2000) Effects of local transcription and H-NS on inversion of the fim switch of Escherichia coli. Mol. Microbiol. 36:457-66 |
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| Abstract |
The fim switch of Escherichia coli is responsible for phase-variable expression of type 1 fimbriae. Switching in the ON-to-OFF and OFF-to-ON directions is promoted by the FimB recombinase, while the FimE recombinase directs switching predominantly in the ON-to-OFF direction. The effects of local promoter activity and the H-NS nucleoid-associated protein on inversion of the switch were assessed. In contrast to FimB-mediated inversion, inversion of the switch by the FimE recombinase was unaffected by the H-NS status of the cell. Transcription towards the switch from within a translationally inactivated fimE gene was found to bias the switch strongly in the OFF direction, creating a FimE+-like phenotype in the absence of the FimE protein. This biasing was H-NS dependent and was also contingent on transcription from within the switch. These data show that local transcription and a nucleoid-associated protein both contribute to the modulation of a site-specific recombination event on the bacterial chromosome. |
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| Keywords |
Alleles; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli Proteins; Fimbriae, Bacterial/genetics; Fimbriae, Bacterial/metabolism; Gene Expression Regulation, Bacterial; Integrases/genetics; Integrases/metabolism; Molecular Sequence Data; Mutagenesis, Site-Directed; Promoter Regions, Genetic/genetics; Recombination, Genetic; Repressor Proteins; Sequence Analysis, DNA; Transcription, Genetic |
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