PMID:10781568
| Citation |
Kwon, O, Georgellis, D, Lynch, AS, Boyd, D and Lin, EC (2000) The ArcB sensor kinase of Escherichia coli: genetic exploration of the transmembrane region. J. Bacteriol. 182:2960-6 |
|---|---|
| Abstract |
The Arc two-component signal transduction system of Escherichia coli regulates the expression of numerous operons in response to respiratory growth conditions. Cellular redox state or proton motive force (Delta(H(+))) has been proposed to be the signal for the membrane-associated ArcB sensor kinase. This study provided evidence for a short ArcB periplasmic bridge that contains a His47. The dispensability of this amino acid, the only amino acid with a pK in the physiological range, renders the Delta(H(+)) model unlikely. Furthermore, results from substituting membrane segments of ArcB with counterparts of MalF indicate that the region does not play a stereospecific role in signal reception. |
| Links | |
| Keywords |
Alkaline Phosphatase; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Cell Membrane/metabolism; Cyclin-Dependent Kinases/genetics; Cyclin-Dependent Kinases/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins; Histidine/genetics; Histidine/metabolism; Membrane Proteins/genetics; Membrane Proteins/metabolism; Protein Kinases/genetics; Protein Kinases/metabolism; Proton-Motive Force/physiology; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism |
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