PMID:10781067

Citation	Herskovits, AA and Bibi, E (2000) Association of Escherichia coli ribosomes with the inner membrane requires the signal recognition particle receptor but is independent of the signal recognition particle. Proc. Natl. Acad. Sci. U.S.A. 97:4621-6
Abstract	In mammalian cells, as well as Escherichia coli, ribosomes translating membrane proteins interact cotranslationally with translocons in the membrane, and this interaction is essential for proper insertion of nascent polypeptides into the membrane. Both the signal recognition particle (SRP) and its receptor (SR) are required for functional association of ribosomes translating integral membrane proteins with the translocon. Herein, we confirm that membrane targeting of E. coli ribosomes requires the prokaryotic SRalpha homolog FtsY in vivo. Surprisingly, however, depletion of the E. coli SRP54 homolog (Ffh) has no significant effect on binding of ribosomes to the membrane, although Ffh depletion is detrimental to growth. These and other observations suggest that, in E. coli, SRP may operate downstream of SR-mediated targeting of ribosomes to the plasma membrane.
Links	PubMed PMC18282 Online version:10.1073/pnas.080077197
Keywords	Arabinose/metabolism; Bacterial Proteins/metabolism; Cell Membrane/metabolism; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Kinetics; Membrane Proteins/genetics; Protein Biosynthesis; Receptors, Cytoplasmic and Nuclear/metabolism; Receptors, Peptide/metabolism; Ribosomes/metabolism; Signal Recognition Particle/metabolism
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